| Complement component | Typing technique | No. of known alleles | Polymorphism
in other species |
| C1q | Imm/Funct | – | – |
| C1r | IEF+WB | > 10 | – |
| C1s | IEF+WB |
2
|
– |
| C2 | IEF+WB
RFLP/PCR |
< 10 | Ch,Rh,Gp,Hm |
| C3 | HVAGE+IFX
RFLP/PCR |
> 30 | Ch,Rh,Ba,Mc,Hl, Ms,Rb,D |
| C4 | HVAGE+IFX/WB
HVAGE+HOV MAB SDS-PAGE (a/b-chains) Imm/HAI (Rodgers/ Chido blood group antigens) RFLP/PCR |
> 30 | Ch,Mac,D,Ms,Gp,Xl |
| C5 | IEF+WB |
2
|
– |
| C6 | IEF+WB | > 20 | Ch,Rh,Rb,Ms |
| C7 | IEF+WB/HOV
MAB |
> 10 | – |
| C8 | IEF+WB/HOV
SDS-PAGE RFLP/PCR |
< 10 | Ch |
| Factor B | HVAGE+IFX/HOV
IEF RFLP/PCR |
> 20 | Ch,Rh,Hl,Ba,Gp,Ms |
| Factor I | IEF+WB
RFLP/PCR |
< 5 | – |
| Factor H | IEF+WB | < 5 | – |
| P | Imm/Funct | – | – |
| C4BP | IEF+WB/IFX | < 5 | – |
| CR1 | SDS-PAGE
EXP RFLP/PCR Imm (Knops blood group antigen) |
< 5 >5 |
– |
| CR2 | SDS-PAGE
RFLP/PCR |
< 2 | – |
| CR3 | SDS-PAGE | – | – |
| DAF | Imm (Cromer
blood group antigen)
RFLP/PCR |
< 2 | – |
| MCP | EXP | < 2 | – |
| MBL | RFLP/PCR |
3
|
– |
| EXP | Expression polymorphism (no. of membrane-associated molecules per cell) | ||||
| Funct | Functional assay | ||||
| HAI | Haemagglutination inhibition with human alloantisera | ||||
| HOV | Complement component-dependent haemolytic overlay | ||||
| HVAGE | High voltage agarose gel electrophoresis | ||||
| IEF | Isoelectric focusing | ||||
| IFX | Immunofixation | ||||
| Imm | Immunological detection with specific antisera | ||||
| MAB | Monoclonal antibodies | ||||
| RFLP/PCR | DNA restriction fragment length polymorphism detected by Southern blot or PCR analysis | ||||
| SDS-PAGE | SDS polyacrylamide gel electrophoresis | ||||
| WB | Western blot | ||||
| Abbreviations of animal species: | Ba:
Baboon; Ch: Chimpanzee; D: dog; Gp: guinea pig; Hm:
hamster;
Hl: Hanuman langur; Mc: Macaque; Ms: mouse; Rb: rabbit; Rh: Rhesus monkey; Xl: Xenopus laevis |
The Complement Genetics Homepage
in Mainz: Complement polymorphism (last update: July 31, 1999)