Complement component | Typing technique | No. of known alleles | Polymorphism
in other species |
C1q | Imm/Funct | – | – |
C1r | IEF+WB | > 10 | – |
C1s | IEF+WB |
2
|
– |
C2 | IEF+WB
RFLP/PCR |
< 10 | Ch,Rh,Gp,Hm |
C3 | HVAGE+IFX
RFLP/PCR |
> 30 | Ch,Rh,Ba,Mc,Hl, Ms,Rb,D |
C4 | HVAGE+IFX/WB
HVAGE+HOV MAB SDS-PAGE (a/b-chains) Imm/HAI (Rodgers/ Chido blood group antigens) RFLP/PCR |
> 30 | Ch,Mac,D,Ms,Gp,Xl |
C5 | IEF+WB |
2
|
– |
C6 | IEF+WB | > 20 | Ch,Rh,Rb,Ms |
C7 | IEF+WB/HOV
MAB |
> 10 | – |
C8 | IEF+WB/HOV
SDS-PAGE RFLP/PCR |
< 10 | Ch |
Factor B | HVAGE+IFX/HOV
IEF RFLP/PCR |
> 20 | Ch,Rh,Hl,Ba,Gp,Ms |
Factor I | IEF+WB
RFLP/PCR |
< 5 | – |
Factor H | IEF+WB | < 5 | – |
P | Imm/Funct | – | – |
C4BP | IEF+WB/IFX | < 5 | – |
CR1 | SDS-PAGE
EXP RFLP/PCR Imm (Knops blood group antigen) |
< 5 >5 |
– |
CR2 | SDS-PAGE
RFLP/PCR |
< 2 | – |
CR3 | SDS-PAGE | – | – |
DAF | Imm (Cromer
blood group antigen)
RFLP/PCR |
< 2 | – |
MCP | EXP | < 2 | – |
MBL | RFLP/PCR |
3
|
– |
EXP | Expression polymorphism (no. of membrane-associated molecules per cell) | ||||
Funct | Functional assay | ||||
HAI | Haemagglutination inhibition with human alloantisera | ||||
HOV | Complement component-dependent haemolytic overlay | ||||
HVAGE | High voltage agarose gel electrophoresis | ||||
IEF | Isoelectric focusing | ||||
IFX | Immunofixation | ||||
Imm | Immunological detection with specific antisera | ||||
MAB | Monoclonal antibodies | ||||
RFLP/PCR | DNA restriction fragment length polymorphism detected by Southern blot or PCR analysis | ||||
SDS-PAGE | SDS polyacrylamide gel electrophoresis | ||||
WB | Western blot |
Abbreviations of animal species: | Ba:
Baboon; Ch: Chimpanzee; D: dog; Gp: guinea pig; Hm:
hamster;
Hl: Hanuman langur; Mc: Macaque; Ms: mouse; Rb: rabbit; Rh: Rhesus monkey; Xl: Xenopus laevis |
The Complement Genetics Homepage
in Mainz: Complement polymorphism (last update: July 31, 1999)